Institute of Anatomy, 1^st Faculty of Charles University, U Nemocnice 3, 128 00 Prague 2
Head: Professor Karel Smetana, MD., DSc. (karel.smetana@lf1.cuni.cz)

Team members: 

Professor Miloš Grim, MD., DSc. (milos.grim@lf1.cuni.cz)
Associated Professor Petr Valášek, MD., PhD. (petr.valasek@lf1.cuni.cz)
Barbora Dvořánková, PhD. (barbora.dvorankova@lf1.cuni.cz)
Zdeněk Fík, MD., PhD. (zdenek.fik@lf1.cuni.cz)
Ivo Klepáček, MD., PhD. (ivo.klepacek@lf1.cuni.cz)
Ondřej Kodet, MD., PhD. (ondrej.kodet@lf1.cuni.cz)
Eliška Krejčí, PhD. (eliska.krejci@lf1.cuni.cz)
Lukáš Lacina, MD., PhD. (lukas.lacina@lf1.cuni.cz)
Pavol Szabo, PhD. (pavol.szabo@lf1.cuni.cz)

Postgraduate students: 

Peter Gál, MSc. (peter.gal@lf1.cuni.cz)
Jan Kučera, MD. (jan.kucera@lf1.cuni.cz)
Rosana Mateu, MSc. (rosana.mateu@lf1.cuni.cz)
Alžběta Mifková, MD. (alzbeta.mifkova@lf1.cuni.cz)
Veronika Živicová, MD. (veronika.zivicova@lf1.cuni.cz)

Technicians:

Marie Jindráková
Radana Kavková, Bc.

Grant projects:

Charles University (PRVOUK-27, UNCE 203407, Specific University Research)
BIOCEV (No. CZ.1.05/1.1.00/02.0109)
GLYCOPHARM (Marie Curie Training Network, Contract No. 317297)
Grant Agency of the Czech Republic (No. 304/12/1333 and 13-20293S)
Grant Agency of Ministry of Health (No. NT-13488-4)

Research activity: Investigation of intercellular crosstalk in malignant tumors and during the wound healing. Our research is focussed predominantly on tumors originated from squamous cell epithelium from (majority cell population such as keratinocytes and minority cells, such as melanocytes). We stress our research activity mainly on the role of cancer-associated fibroblasts including definition of their expression profiles. We collaborate with Department of Otorhinolaryngology and Head and Neck Surgery, Department of Dermatovenerology and Department of Biochemistry and Experimental Oncology of the 1st Faculty of Medicine of the Charles University in Prague, Institute of Molecular Genetics of the Academy of Sciences of the Czech Republic vvi. in Prague, and Institut de Sciences et d’Ingenierie Supramoleculaires (ISIS) of the University of Strasbourg.

Technologies

Cell /tissue culture
Histology/quantitative microscopy
Immunocytochemistry
Immunohistochemistry
Enzyme-histochemistry
Lectinhistochemistry (normal and reversed)
ELISA, MTT-test

Equipment

Cell /tissue culture laboratory equipped with flow boxes and CO2 incubators
Fluorescence microscope (Nikon, Eclipse) with computer assisted image analysis (LUCIA)
Inverted fluorescence microscope (Olympus)
Cryocut (Reichert)

Main selected publications:

Jarkovska, K., Dvorankova, B., Halada, P., Kodet, O., Szabo, P., Gadher S., J., Motlik, J., Kovarova, H., Smetana, K., Jr.: Revelation of fibroblast protein commonalities and differences and their possible roles in wound healing and tumourigenesis using co culture models of cells. Biol. Cell 106: 203–218 (2014)
http://www.ncbi.nlm.nih.gov/pubmed/24698078

Kodet O., Lacina L, Krejčí E., Dvořánková B., Grim M., Štork J., Kodetová D., Vlček Č., Šáchová J., Kolář M., Strnad H., Smetana K. Jr.: Melanoma cells influence the differentiation pattern of human epidermal keratinocytes. Mol. Cancer 14: 1 (2015)
http://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-14-1

Kopitz, J., Fík, Z., André, S., Smetana, K., Jr., Gabius, H.-J.: Single-site mutational engineering and following mono PEGylation of the huma lectin galectin-2: Effect on ligand binding, functional aspects and clearance from serum. Mol. Pharmaceutics 10: 2054-2061 (2013)
http://www.ncbi.nlm.nih.gov/pubmed/23581621

Solís, D., Bovin, N.V., Davis, A.P., Jiménez-Barbero, J., Romero, A., Roy, R., Smetana, K. Jr., Gabius, H.-J..: A guide into glycosciences: How chemistry, biochemistry and biology cooperate to crack the sugar code. Biochim Biophys Acta. 1850: 186-235 (2015)
http://www.ncbi.nlm.nih.gov/pubmed/24685397

Trylcova, J., Busek, P., Smetana, K. Jr., Balaziova, E., Dvorankova, B., Mifkova A., Sedo, A.: Effect of cancer-associated fibroblasts on the migration of glioma cells in vitro. Tumor Biol
http://www.ncbi.nlm.nih.gov/pubmed/25712375

Valach J, Fík Z, Strnad H, Chovanec M, Plzák J, Čada Z, Szabó P, Šáchová J, Hroudová M, Urbanová M, Šteffl M, Pačes J, Mazánek J, Vlček Č, Betka J, Kaltner H, André S, Gabius H-J, Kodet R, Smetana K Jr, Gál P, Kolář M. Smooth muscle actin-expressing stromal fibroblasts in head and neck squamous cell carcinoma: increased expression of galectin-1 and induction of poor-prognosis factors. Int J Cancer 131: 2499-2508 (2012)
http://onlinelibrary.wiley.com/doi/10.1002/ijc.27550/pdf