Neurooncolgy group includes reserchers and surgeons from the Laboratory of cancer cell biology (LCCB) and the Clinic of Neurosurgery and Neuro-Oncology (CNNO), respectively. It focuses mostly on the role of proteases in gliomagenesis and in limited extent also in tumorigenesis of another solid tumors. Previous work of the LCCB contributed to the definition of the group of „Dipeptidylpeptidase-IV activity and/or structure homologues (DASH) “and elucidated the expression of these proteases in brain tumors. Current projects include studies on the mechanisms by which DASH molecules present in the tissue microenvironment contribute to the progression of tumors originating from glial cells and also pancreatic cancer. Some of the DASH molecules are also evaluated as possible molecular targets for advanced therapeutic interventions. The Group is also a founding member of the Neurooncological section of the Czech Society for Oncology of the Czech Medical Association of J.E. Purkyně.



Web page: www.lf1.cuni.cz/lbnb

Team:
Professor Aleksi Sedo, M.D., Ph.D.- leader (LBNB)
Professor Vladimir Beneš, M.D., Ph.D. (CNNB)
Assistant professor David Netuka M.D., PhD (CNNB)
Assistant professor Evzen Krepela, M.D., Ph.D. (LBNB)
Filip Kramář, MD., PhD (CNNB)
Ondrřej Bradáč, M.D., MSc. (CNNB)
Petr Busek, M.D., Ph.D. (LBNB)
Eva Balaziova, M.D., Ph.D. (LBNB)

Ph.D. students
Marek Hilser, M.D.
Zdislava Vanickova, MD
Lucie Sromova, MSc
Edita Fejfarova, MSc
Jana Trylcova, MSc
Ivana Matrasova, MSc

Technicians
Kvetoslava Vlasicova
Karin Roubickova

Selected publications:
Co-expression of the homologous proteases fibroblast activation protein and dipeptidyl peptidase-IV in the adult human Langerhans islets. Busek P, Hrabal P, Fric P, Sedo A.
Histochem Cell Biol. 2015 May;143(5):497-504. (IF2013=2.93)

Effect of cancer-associated fibroblasts on the migration of glioma cells in vitro.
Trylcova J, Busek P, Smetana K Jr, Balaziova E, Dvorankova B, Mifkova A, Sedo A.
Tumour Biol. 2015 (in press) (IF2013=2.84)

Glioma–associated proteases. Busek P, Prevorovsky M, Krepela E, Sedo A. In: Glioma Cell Biology, Editors: Sedo A, Mentlein R, Springer 2014, ISBN 978-3-7091-1431-5

Dipeptidyl peptidase-IV inhibits glioma cell growth independent of its enzymatic activity.
Busek P, Stremenova J, Sromova L, Hilser M, Balaziova E, Kosek D, Trylcova J, Strnad H, Krepela E, Sedo A. Int J Biochem Cell Biol. 2012 May;44(5):738-47. (IF2013=4.24)

Coupled expression of dipeptidyl peptidase-IV and fibroblast activation protein-α in transformed astrocytic cells. Balaziova E, Busek P, Stremenova J, Sromova L, Krepela E, Lizcova L, Sedo A. Mol Cell Biochem. 2011 Aug;354(1-2):283-9. (IF2013=2.39)

Expression of dipeptidyl peptidase-IV activity and/or structure homologs in human meningiomas. Stremenova J., Mares V., Lisa V., Hilser M., Krepela E., Vanickova Z., Syrucek M., Soula O., Sedo A.: Int J Oncol. 2010 36(2): 351-8. (IF2013=2.77)

Technologies

• Cell biology (in vitro cell culture; derivation of primary cell cultures and glioma stem-like cell; transfection; cell growth, migration and invasion assays; transwell co-culture; angiogenesis assays)
• Immunohistochemistry, epifluorescence and laser scanning confocal microscopy, time lapse imaging
• Expression analysis by qPCR
• In vivo tumor models (intracranial and subcutaneous xenotransplants, genetically engineered mouse models, chorioallantoic membrane assay)
• Flow cytometry, cell cycle analysis
• Enzyme activity and inhibitor assays, ELISA, isoelectric focusing, 2D electrophoresis
  

Equipment

• PCR cycler; equipment for electrophoresis, protein 2D electrophoresis, isoelectric focusing, WB
• Spectrofluorometer Fluoromax 4, Perkin Elmer multiwell plate reader Victor3, Tecan Sunrise ELISA reader
• Flow boxes, CO2 incubators, hypoxic chamber
• Laser scanning confocal microscope Olympus FV300, fluorescence microscope
• Equipment for stereotactic intracranial xenotransplantation
• Flow cytometer BD FACS Verse
• Leica cryotome CM1950, Leica vibratome V1200, Leica microdissector CTR 6000